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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were being documented but in depth nucleotide sequence information and facts is just not however readily available.

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Blocking peptides are peptides that bind exclusively for the target antibody and block antibody binding. These peptide ordinarily incorporates the epitope identified through the antibody.

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This information outlines more attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best effects are...

Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is precise.

Focus on Information Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic celebration. Cyclin E types a complex with and functions being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development by means of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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